Quantitative techniques such as UV-Vis Spectroscopy, also known as Spectrophotometry, are utilized to determine the extent to which a chemical substance absorbs light. This is accomplished by comparing the amount of light that travels through a sample to the amount of light that travels through a reference sample, also known as a blank. This methodology is applicable to a wide variety of sample types, including liquids, solids, thin films, and even glass.

Spectrophotometers with high performance from Edinburgh Instruments are suitable for a wide variety of analytical applications, including those in which accurate and precise measurements are essential to achieving the desired results.

A quantitative measurement of the absorption, transmission, or reflection of a material as a function of wavelength is referred to as spectrophotometery. In spite of the fact that vis spectrophotometer (see more) is referred to as UV-Vis, the wavelength range that is typically utilized begins at 190 nm and extends all the way into the near-infrared at 1,100 nm.

By using a spectrophotometer and carrying out absorption/transmission measurements, we are able to simply determine the amount (or concentration) of a known chemical substance by analyzing the number of photons (the intensity of the light) that reach the detector. This is done with the help of an absorption/transmission curve. When a material absorbs light at a particular wavelength to a greater extent, the concentration of the known substance in the material is increased.

Ultraviolet-visible spectrophotometry, also known as UV-Vis spectrophotometry, is a method that is used to measure the amount of light that is absorbed across the ultraviolet and visible ranges of the electromagnetic spectrum. When light from an external source strikes a material object, the light can either be absorbed, reflected, or transmitted. Atomic excitation is caused when radiation in the UV-Vis range is absorbed. This process describes the movement of molecules from a ground state with a low amount of energy to a state with a higher amount of energy.

It is necessary for an atom to take in sufficient levels of radiation so that its electrons can move into higher molecular orbits before the atom can change its excitation state. In general, the absorption of shorter wavelengths of light is correlated with bandgaps that are shorter. Therefore, the amount of energy that molecules need to expend in order to go through these transitions is electrochemically-specific. This principle can be applied by a UV-Vis spectrophotometer, which allows the analytes in a sample to be quantified based on the characteristics of their absorption.

Spectrophotometers that Measure UV-Vis Absorption and Emission
A UV-Vis spectrophotometer compares a measurement of the intensity of light that was incident on a sample to a reference measurement of the intensity of light that was transmitted through the sample. The well-known DS-11 series of UV-Vis spectrophotometers manufactured by Drawellanalytical makes use of a pulsed Xenon flash lamp to illuminate microvolume samples with light that spans a wavelength range of 190 nm to 840 nm. A CCD optical detector that has a wavelength accuracy of within 0.5 nm acquires the transmitted light so that it can be analyzed.

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According to the Beer-Lambert Law, the amount of light that is absorbed is directly proportional to the concentration of the sample as well as the pathlength, which refers to the distance that light travels through the sample.

Controlling the analysis wavelengths and the pathlength allows UV-Vis spectrophotometers to calculate the concentration of individual analytes contained within a microvolume. Any other UV-Vis spectrophotometer that is currently on the market cannot compare to the DS-11's SmartPath®Technology, which enables accurate measurements of samples at higher concentrations than any other UV-Vis spectrophotometer on the market. This method utilizes real-time absorbance to determine the optimal path length for each sample and then makes automatic adjustments to tailor itself to the detection range of the equipment.

The DS-11 series is capable of performing microvolume measurements on samples with a minimum volume of 0.5 microliters (L) and can utilize either quartz or plastic cuvettes. After that, the concentration, full spectral output, and sample purity of proteins, single- and double-stranded DNA (ssDNA and dsDNA), and ribonucleic acid (RNA) can be rapidly and automatically evaluated.

Spectrophotometers that measure UV-Vis Absorption and Emission from Drawellanalytical
Drawellanalytical offers a variety of UV-Vis spectrophotometers from their DS-11 series. These spectrophotometers feature unrivaled precision and the widest dynamic range that is technically possible. Measurement modes for fluorescence and absorbance in microvolume and cuvette formats are included in the models.

Read our earlier post titled "The Working Principle of a Fluorometer" if you are interested in learning more about the technologies that we have available for purchase. You can also get in touch with us directly if you have any inquiries concerning the DS-11 UV-Vis spectrophotometer that we sell.